Cytochrome P450 1A2 Antibody (D15) - BSA Free

Novus Biologicals | Catalog # NB600-1490

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # D15

Format

BSA Free
View all Cytochrome P450 1A2 Primary Antibodies »

Product Specifications

Immunogen

MC1a (Preparation C31B4, rat liver cytochrome P4501A2)

Localization

Liver endoplasmic reticulum, membrane bound.

Specificity

D15 cross reacts with CYP1A1 & CYP1A2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Cytochrome P450 1A2 Antibody (D15) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): MCF7 cells (ICC/IF) or human normal liver (IHC).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome P450 1A2

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Long Name

Cytochrome P450 1A2

Alternate Names

CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3

Entrez Gene IDs

1544 (Human); 13077 (Mouse); 24297 (Rat)

Gene Symbol

CYP1A2

Additional Cytochrome P450 1A2 Products

Product Documents for Cytochrome P450 1A2 Antibody (D15) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 1A2 Antibody (D15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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