Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44263

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14565

Format

Azide and BSA Free
View all Cytokeratin 14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P02533, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free

Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Staining of human breast shows strong cytoplasmic positivity in basal layer of ductal cells.
Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Analysis in human skin and testis tissues using NBP3-44263 antibody. Corresponding Cytokeratin 14 RNA-seq data are presented for the same tissues.
Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody [NBP3-44263]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody [NBP3-44263]

Staining in A-431 cell line with Anti-Cytokeratin 14 monoclonal antibody).
Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Staining of human prostate shows strong cytoplasmic positivity in basal layer of glandular cells.
Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP3-44263]

Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free Western Blot: Cytokeratin 14 Antibody [NBP3-44263]

Western Blot: Cytokeratin 14 Antibody [NBP3-44263]

Analysis in human cell line U-2197.

Applications for Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytokeratin 14

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

Additional Cytokeratin 14 Products

Product Documents for Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 14 Antibody (CL14565) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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