Cytokeratin 15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85602

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 15 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 15 Antibody - BSA Free

Western Blot: Cytokeratin 15 Antibody [NBP1-85602]

Western Blot: Cytokeratin 15 Antibody [NBP1-85602]

Western Blot: Cytokeratin 15 Antibody [NBP1-85602] - Analysis in human cell line A-431 and human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody [NBP1-85602]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody [NBP1-85602]

Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human cell line HaCaT shows localization to nucleoplasm & intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human kidney shows no positivity in cells in tubules as expected.
Cytokeratin 15 Antibody - BSA Free Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody - BSA Free [NBP1-85602]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody - BSA Free [NBP1-85602]

Immunohistochemistry analysis in human skin and kidney tissues using HPA024554 antibody. Corresponding KRT15 RNA-seq data are presented for the same tissues.

Applications for Cytokeratin 15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 15

Long Name

keratin 15

Alternate Names

CK15, CK-15, cytokeratin 15, cytokeratin-15, K15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15

Gene Symbol

KRT15

Additional Cytokeratin 15 Products

Product Documents for Cytokeratin 15 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 15 Antibody - BSA Free

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Protocols

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FAQs

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