Cytokeratin 7/13/17/18 Antibody (ST50-05)

Novus Biologicals | Catalog # NBP2-67533

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST50-05 expressed in HEK293
View all Cytokeratin 7/13/17/18 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Cytokeratin 7/13/17/18 aa 18-67. (SwissProt: P08729 Human; SwissProt: Q9DCV7 Mouse)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 7/13/17/18 Antibody (ST50-05)

Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: HepG2 Lane 3: SK-BR-3 Lane 4: SW1990
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of SKBR-3 cells labeling Cytokeratin 7. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with the primary antibodies Cytokeratin 7 (red) at 1/100 dilution for overnight at 4. Goat anti rabbit IgG (iFluorTM 594) was used as the secondary antibody at 1/1,000 dilution dilution. DAPI was used as nuclear counterstain.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 ( washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Flow cytometric analysis of Cytokeratin 7/13/17/18 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cytokeratin 7/13/17/18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 7/13/17/18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human placenta tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533]

Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Hela cells with Cytokeratin 7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Applications for Cytokeratin 7/13/17/18 Antibody (ST50-05)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 7/13/17/18

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue- and differentiation-specific expression profiles. Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19. Keratin 17 is expressed in suprabasal keratinocytes of wounded epidermis. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. LdS 23 is useful for studies of epithelial cells and tumour origin.

Alternate Names

CK7, CK-7, cytokeratin 7, cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, Keratin-7, MGC129731, MGC3625, sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbol

KRT7

Additional Cytokeratin 7/13/17/18 Products

Product Documents for Cytokeratin 7/13/17/18 Antibody (ST50-05)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 7/13/17/18 Antibody (ST50-05)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 7/13/17/18 Antibody (ST50-05)

There are currently no reviews for this product. Be the first to review Cytokeratin 7/13/17/18 Antibody (ST50-05) and earn rewards!

Have you used Cytokeratin 7/13/17/18 Antibody (ST50-05)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies