DAP3 Antibody

Novus Biologicals | Catalog # NB100-1032

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence NPSLLERHCAYL corresponding to C-Terminus according to NP_387506.1, NP_001186779.1, NP_001186780.1.

Localization

Mitochondrial

Specificity

This antibody is expected to recognize isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DAP3 Antibody

Western Blot: DAP3 Antibody [NB100-1032]

Western Blot: DAP3 Antibody [NB100-1032]

Western Blot: DAP3 Antibody [NB100-1032] - Staining (0.3 ug/mL) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NB100-1032]

Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NB100-1032]

Immunocytochemistry/Immunofluorescence: DAP3 Antibody [NB100-1032] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Western Blot: DAP3 Antibody [NB100-1032]

Western Blot: DAP3 Antibody [NB100-1032]

Western Blot: DAP3 Antibody [NB100-1032] - Staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
DAP3 Antibody

Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] -

Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
DAP3 Antibody

Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] -

Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for DAP3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 1 ug/mL
Application Notes
WB: Approx 40 kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41 kDa according to NP_001186779.1 and NP_001186780). Primary incubated 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAP3

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Alternate Names

bMRP-10, DAP-3Death-associated protein 3, death associated protein 3, DKFZp686G12159,28S ribosomal protein S29, mitochondrial, FLJ12817, MGC126058, MGC126059, mitochondrial 28S ribosomal protein S29, MRP-S29Ionizing radiation resistance conferring protein, MRPS29S29mt

Entrez Gene IDs

7818 (Human)

Gene Symbol

DAP3

UniProt

NP_387506.1 (Human), NP_001186779.1 (Human), NP_001186780.1 (Human)

Additional DAP3 Products

Product Documents for DAP3 Antibody

Certificate of Analysis

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Product Specific Notices for DAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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