Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDIT4 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 12 amino acid synthetic peptide from near the center of human RTP801. The immunogen is located within amino acids 90 - 140 of RTP801. Amino Acid Squence: QARLGSRRPARL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit DDIT4 Antibody - BSA Free (NBP1-77321) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-DDIT4 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DDIT4 Antibody - BSA Free

Knockdown Validated: DDIT4 Antibody - BSA Free [NBP1-77321]

Western Blot: DDIT4 Antibody - BSA Free [NBP1-77321]

DDIT4-Antibody-Knockdown-Validated-NBP1-77321-img0009.jpg
Western Blot: DDIT4 AntibodyBSA Free [NBP1-77321]

Western Blot: DDIT4 AntibodyBSA Free [NBP1-77321]

DDIT4-Antibody-Western-Blot-NBP1-77321-img0010.jpg
Western Blot: DDIT4 AntibodyBSA Free [NBP1-77321]

Western Blot: DDIT4 AntibodyBSA Free [NBP1-77321]

Western Blot: DDIT4 Antibody [NBP1-77321] - Human kidney tissue lysate with DDIT4 antibody at (A) 0.5 and (B) 1 ug/mL.
DDIT4 Antibody - BSA Free

Immunohistochemistry: DDIT4 Antibody - BSA Free [NBP1-77321] -

Immunohistochemistry: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunohistochemistry of DDIT4 in mouse kidney tissue with DDIT4 antibody at 5 u/mL.
DDIT4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321] -

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321] - Immunofluorescence of DDIT4 in mouse kidney tissue with DDIT4 antibody at 20 ug/mL.

Red: DDIT4 Antibody
Blue: DAPI staining

Applications for DDIT4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDIT4

RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.

Alternate Names

Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1

Gene Symbol

DDIT4

UniProt

NP_061931 (Human)

Additional DDIT4 Products

Product Documents for DDIT4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DDIT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDIT4 Antibody - BSA Free

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Protocols

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