DDX3 Antibody (3B9E3) - BSA Free

Novus Biologicals | Catalog # NBP2-52457

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Monkey

Cited:

Mouse, Hamster

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3B9E3

Format

BSA Free
View all DDX3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human DDX3 (AA: 518-661) expressed in E. Coli.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 28516729).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

73.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX3 Antibody (3B9E3) - BSA Free

Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.
Immunohistochemistry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunohistochemistry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
Flow Cytometry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Flow Cytometry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).
Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.
Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3)BSA Free [NBP2-52457]

Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)
Immunohistochemistry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunohistochemistry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.
Flow Cytometry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Flow Cytometry: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).
Immunofluorescence: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunofluorescence: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunofluorescence: DDX3 Antibody (3B9E3) - BSA Free [NBP2-52457]

Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Applications for DDX3 Antibody (3B9E3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry

1:200-1:1000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready. Western blot reported from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4 using NBP2-52457 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX3

DDX3X, also known as ATP-dependent RNA helicase DDX3X, consists of a 73.2 kDa and a 71.4 kDa isoform which is involved in multiple gene expression steps, including transcription and translation. Current research is linking the protein to diseases and disorders such as hypoxia, hepatitis, malaria, infertility, carcinoma, breast cancer, and prostate cancer. The protein interacts in translational control, cell cycle checkpoint control, and receptor signaling pathways with other proteins such as ACTB, TBK1, NFKB2, NXF1, and EIF3B.

Alternate Names

DBXCAP-Rf, DDX14, DDX3DEAD/H box-3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, EC 3.6.1, EC 3.6.4.13, helicase like protein 2, Helicase-like protein 2, HLP2ATP-dependent RNA helicase DDX3X

Gene Symbol

DDX3X

Additional DDX3 Products

Product Documents for DDX3 Antibody (3B9E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DDX3 Antibody (3B9E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDX3 Antibody (3B9E3) - BSA Free

Customer Reviews for DDX3 Antibody (3B9E3) - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • DDX3 Antibody (3B9E3)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: hela whole cell lysate
    Species: Human
    Verified Customer | Posted 06/21/2018
    DDX3 Antibody (3B9E3) - BSA Free NBP2-52457
  • DDX3 Antibody (3B9E3)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: the baby hamster kidney cell line (BHK-21)
    Species: the baby hamster kidney cell line (BHK-21)
    Verified Customer | Posted 12/13/2017
    Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control BHK-21 cells
    DDX3 Antibody (3B9E3) - BSA Free NBP2-52457

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Protocols

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