DDX3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85291

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX3 Antibody - BSA Free

Western Blot: DDX3 Antibody [NBP1-85291]

Western Blot: DDX3 Antibody [NBP1-85291]

Western Blot: DDX3 Antibody [NBP1-85291] - Analysis: NBP1-85291 (A) shows similar pattern to independent antibody NBP1-85290 (B)
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP1-85291]

Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP1-85291]

Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-85291] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse hippocampus shows moderate positivity in a subset of neurons in the brain.
Western Blot: DDX3 Antibody [NBP1-85291]

Western Blot: DDX3 Antibody [NBP1-85291]

Western Blot: DDX3 Antibody [NBP1-85291] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse medulla shows strong cytoplasmic immunoreactivity in neurons of the facial nucleus.
Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse thalamus shows positivity in neurons of the anterodorsal thalamic nucleus.
Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse midbrain shows neuronal positivity in the red nucleus.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear immunoreactivity in neurons.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human hippocampus shows moderate positivity in neurons.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human Fallopian tube shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291]

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear positivity in neurons.
Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291]

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse cerebral cortex shows strong positivity in neurons in the parietal association cortex.

Applications for DDX3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX3

DDX3X, also known as ATP-dependent RNA helicase DDX3X, consists of a 73.2 kDa and a 71.4 kDa isoform which is involved in multiple gene expression steps, including transcription and translation. Current research is linking the protein to diseases and disorders such as hypoxia, hepatitis, malaria, infertility, carcinoma, breast cancer, and prostate cancer. The protein interacts in translational control, cell cycle checkpoint control, and receptor signaling pathways with other proteins such as ACTB, TBK1, NFKB2, NXF1, and EIF3B.

Alternate Names

DBXCAP-Rf, DDX14, DDX3DEAD/H box-3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, EC 3.6.1, EC 3.6.4.13, helicase like protein 2, Helicase-like protein 2, HLP2ATP-dependent RNA helicase DDX3X

Gene Symbol

DDX3X

Additional DDX3 Products

Product Documents for DDX3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DDX3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDX3 Antibody - BSA Free

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Protocols

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FAQs

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