DDX5 Antibody

Novus Biologicals | Catalog # NB100-1410

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DDX5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PMIGYPMPTGYSQ corresponding to C-Terminus according to NP_004387.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DDX5 Antibody

Western Blot: DDX5 Antibody [NB100-1410]

Western Blot: DDX5 Antibody [NB100-1410]

Western Blot: DDX5 Antibody [NB100-1410] - (0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DDX5 Antibody [NB100-1410]

Immunohistochemistry-Paraffin: DDX5 Antibody [NB100-1410]

Immunohistochemistry-Paraffin: DDX5 Antibody [NB100-1410] - Paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.
Western Blot: DDX5 Antibody [NB100-1410]

Western Blot: DDX5 Antibody [NB100-1410]

Western Blot: DDX5 Antibody [NB100-1410] - DDX5 / p68 RNA helicase in Hela lysate using this antibody at 0.5 ug/ ml.

Applications for DDX5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2-5 ug/ml

Peptide ELISA

Detection Limit 1:8000

Western Blot

0.1-0.3 ug/ml
Application Notes
IF: An anonymous customer has reported successful staining on HT-1080 cells with a Rhodamine conjugated secondary antibody. Data obtained from a previous batch (different goat). WB: Approx. 70 kDa band observed in nuclear lysates of NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1 kDa band according to NP_004387.1). IHC-P: Human prostate shows nuclear staining of the secretory cells in the glands. Data obtained from a previous batch (different goat).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDX5

DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Long Name

DEAD Box Protein 5

Alternate Names

DEAD/H, G17P1, HELR, HLR1, HUMP68

Entrez Gene IDs

1655 (Human); 13207 (Mouse)

Gene Symbol

DDX5

UniProt

NP_004387. (Human)

Additional DDX5 Products

Product Documents for DDX5 Antibody

Certificate of Analysis

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Product Specific Notices for DDX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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