Desmin Antibody (CL4502) - BSA Free
Novus Biologicals | Catalog # NBP2-59056
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL4502
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P17661, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Desmin Antibody (CL4502) - BSA Free
Western Blot: Desmin Antibody (CL4502) [NBP2-59056]
Western Blot: Desmin Antibody (CL4502) [NBP2-59056] - Analysis in human cell line RH-30.Immunocytochemistry/ Immunofluorescence: Desmin Antibody (CL4502) [NBP2-59056]
Immunocytochemistry/Immunofluorescence: Desmin Antibody (CL4502) [NBP2-59056] - Staining of MCF7 cells showing no specific staining of intermediate filaments. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).Immunocytochemistry/ Immunofluorescence: Desmin Antibody (CL4502) [NBP2-59056]
Immunocytochemistry/Immunofluorescence: Desmin Antibody (CL4502) [NBP2-59056] - Staining of RH-30 cells showing specific staining of intermediate filaments in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).Applications for Desmin Antibody (CL4502) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Western Blot
1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Desmin
Alternate Names
CMD1I, DES
Gene Symbol
DES
Additional Desmin Products
Product Documents for Desmin Antibody (CL4502) - BSA Free
Certificate of Analysis
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Product Specific Notices for Desmin Antibody (CL4502) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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