Desmin Antibody

Novus Biologicals | Catalog # NBP1-06984

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Desmin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RDGEVVSEATQQQHE corresponding to C-Terminus according to NP_001918.3.

Epitope

C Terminus

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25575786).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Desmin Antibody

Desmin Antibody

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-06984] -

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-06984] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Desmin Antibody

Western Blot: Desmin Antibody [NBP1-06984] -

Western Blot: Desmin Antibody [NBP1-06984] - (0.01ug/ml) staining of Human Skeletal Muscle (A) and (0.3ug/ml) Heart (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Desmin Antibody

Western Blot: Desmin Antibody [NBP1-06984] -

Western Blot: Desmin Antibody [NBP1-06984] - (0.1ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Desmin Antibody

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-06984] -

Immunohistochemistry-Paraffin: Desmin Antibody [NBP1-06984] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Applications for Desmin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

4-6 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01-0.3 ug/ml
Application Notes
Western blot: Approx. 55-60kDa band observed in Human and Mouse Skeletal Muscle lysates and in Human Heart lysates (calculated MW of 53.5kDa according to Human NP_001918.3, and Mouse NP_034173.1). Preliminary testing showed a band at approx 60kDa in Rat and Pig Skeletal muscle lysate. Primary incubation 1 hour at room temperature. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 25575786).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Desmin

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]

Alternate Names

CMD1I, DES

Entrez Gene IDs

1674 (Human); 13346 (Mouse); 64362 (Rat)

Gene Symbol

DES

UniProt

NP_001918.3 (Human)

Additional Desmin Products

Product Documents for Desmin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Desmin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers

Citations for Desmin Antibody

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Protocols

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