Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all DGAT1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region (within residues 200-300) of human DGAT1. [Swiss-Prot# O75907]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32326330).

Localization

Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for DGAT1 Antibody [FITC]

Application
Recommended Usage

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DGAT1

Anabolic signals such as glucose, insulin etc. enhance the transcription of lipogenic enzymes responsible for converting excessive carbohydrates into triglycerides, whereas, DGAT1 (diacylglycerol O-acyltransferase 1) is an enzyme that facilitates the conversion of fatty acids to triglycerides. DGAT1 is a multi-pass membrane protein localized in endoplasmic reticulum which catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2, it is not essential for survival, and is involved in VLDL assembly. In liver, DGAT1 plays a key role in esterifying exogenous fatty acids to glycerol, and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. Moreover, it acts as the major acyl-CoA retinol acyltransferase (ARAT) in skin tissues, wherein, it maintains the retinoid homeostasis for preventing retinoid toxicity responsible for skin and hair disorders.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Gene Symbol

DGAT1

Additional DGAT1 Products

Product Documents for DGAT1 Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DGAT1 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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