DGAT1 Antibody - BSA Free

Novus Biologicals | Catalog # NB110-41487

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Inhibition Activity

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DGAT1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region (within residues 200-300) of human DGAT1. [Swiss-Prot# O75907]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32326330).

Localization

Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGAT1 Antibody - BSA Free

Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.
Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NB110-41487]

Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NB110-41487]

Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-DGAT1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

Western Blot: DGAT1 AntibodyBSA Free [NB110-41487]

DGAT1-Antibody-Western-Blot-NB110-41487-img0004.jpg

Applications for DGAT1 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 23668758)

Immunocytochemistry/ Immunofluorescence

1:40-1:100

Simple Western

1:50

Western Blot

2 ug/ml
Application Notes
In Western Blot, a band is seen at ~55 kDa. In ICC/IF, staining was observed in the endoplasmic reticulum and cytoplasm of HepG2 cells. This antibody is not applicable for IHC-paraffin embedded sections.

Reviewed Applications

Read 1 review rated 3 using NB110-41487 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DGAT1

Anabolic signals such as glucose, insulin etc. enhance the transcription of lipogenic enzymes responsible for converting excessive carbohydrates into triglycerides, whereas, DGAT1 (diacylglycerol O-acyltransferase 1) is an enzyme that facilitates the conversion of fatty acids to triglycerides. DGAT1 is a multi-pass membrane protein localized in endoplasmic reticulum which catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2, it is not essential for survival, and is involved in VLDL assembly. In liver, DGAT1 plays a key role in esterifying exogenous fatty acids to glycerol, and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. Moreover, it acts as the major acyl-CoA retinol acyltransferase (ARAT) in skin tissues, wherein, it maintains the retinoid homeostasis for preventing retinoid toxicity responsible for skin and hair disorders.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Gene Symbol

DGAT1

Additional DGAT1 Products

Product Documents for DGAT1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DGAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for DGAT1 Antibody - BSA Free

Customer Reviews for DGAT1 Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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  • Name: Zachary Patinkin
    Application: Simple Western
    Sample Tested: Mouse skeletal muscle homogenate
    Species: Mouse
    Verified Customer | Posted 07/22/2015
    0.2 ug/uL mouse skeletal muscle tissue homogenate. Specific band ~50 kDa.
    DGAT1 Antibody - BSA Free NB110-41487

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Protocols

View specific protocols for DGAT1 Antibody - BSA Free (NB110-41487):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DGAT1 Antibody - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Do you offer the blocking peptide for NB110-41487?

    A: Unfortunately, we do not offer the blocking peptide for NB110-41487. I apologize for any inconvenience.

  • Q: Have you ever tested if DGAT1 NB110-41487 work for rat liver tissues?

    A: We have not specifically tested rat liver tissue, but rat is a species covered by our guarantee for this product.

  • Q: I would like to order your anti-DGAT1 antibody NB110-41487. The immunogen for this is a synthetic peptide designed within residues 200-300 of human DGAT1. I would like to use it to detect the homolog of DGAT1 in Drosophila. I know that the sequence is proprietary but could you tell me if the sequence is conserved in Drosophila (that means that the peptide is designed within residues 243-288 of human DGAT1) so that it could work in flies.

    A: Unfortunately, the Drosophila and human DGAT1 sequences are too divergent to recommend that NB110-41487 may cross-react with fly.

  • Q: Do you offer the blocking peptide for NB110-41487?

    A: Unfortunately, we do not offer the blocking peptide for NB110-41487. I apologize for any inconvenience.

  • Q: Have you ever tested if DGAT1 NB110-41487 work for rat liver tissues?

    A: We have not specifically tested rat liver tissue, but rat is a species covered by our guarantee for this product.

  • Q: I would like to order your anti-DGAT1 antibody NB110-41487. The immunogen for this is a synthetic peptide designed within residues 200-300 of human DGAT1. I would like to use it to detect the homolog of DGAT1 in Drosophila. I know that the sequence is proprietary but could you tell me if the sequence is conserved in Drosophila (that means that the peptide is designed within residues 243-288 of human DGAT1) so that it could work in flies.

    A: Unfortunately, the Drosophila and human DGAT1 sequences are too divergent to recommend that NB110-41487 may cross-react with fly.

  • Q: Do you offer the blocking peptide for NB110-41487?

    A: Unfortunately, we do not offer the blocking peptide for NB110-41487. I apologize for any inconvenience.

  • Q: Have you ever tested if DGAT1 NB110-41487 work for rat liver tissues?

    A: We have not specifically tested rat liver tissue, but rat is a species covered by our guarantee for this product.

  • Q: I would like to order your anti-DGAT1 antibody NB110-41487. The immunogen for this is a synthetic peptide designed within residues 200-300 of human DGAT1. I would like to use it to detect the homolog of DGAT1 in Drosophila. I know that the sequence is proprietary but could you tell me if the sequence is conserved in Drosophila (that means that the peptide is designed within residues 243-288 of human DGAT1) so that it could work in flies.

    A: Unfortunately, the Drosophila and human DGAT1 sequences are too divergent to recommend that NB110-41487 may cross-react with fly.

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