Digoxigenin Antibody (611532)

R&D Systems | Catalog # MAB75201

R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 611532
View all Digoxigenin Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Digoxigenin

Specificity

Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Digoxigenin Antibody (611532)

Detection of Digoxigenin antibody by Western Blot.

Detection of Digoxigenin by Western Blot.

Western blot shows lysates of Goat IgG labeled with Digoxigenin. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB75201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IgG labeled with Digoxigenin at approximately 25 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Digoxigenin Antibody (611532)

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Goat IgG labeled with Digoxigenin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References

  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Antibody (611532)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Digoxigenin Antibody (611532)

For research use only

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