Detection of Digoxigenin by Western Blot. Western blot shows lysates of Goat IgG labeled with Digoxigenin. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB75201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IgG labeled with Digoxigenin at approximately 25 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.
Décarie, A.A. et al. (1994) Peptides 15(3):511.
Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
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