DIO2 Antibody

Novus Biologicals | Catalog # NBP1-00178

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Sheep

Cited:

Human, Mouse, Ovine

Predicted:

Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DIO2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence EVKKHQNQEDRC corresponding to internal region according to NP_054644.1, NP_001007024.1.

Reactivity Notes

Use in Sheep reported in secitific publication PMID: 32403966

Specificity

This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DIO2 Antibody

Immunocytochemistry/ Immunofluorescence: DIO2 Antibody [NBP1-00178]

Immunocytochemistry/ Immunofluorescence: DIO2 Antibody [NBP1-00178]

Immunocytochemistry/Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178]

Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178]

Immunohistochemistry-Frozen: DIO2 Antibody [NBP1-00178] - IHC analysis of DIO2 in cryo-sectioned Mouse Hippocampus using NBP1-00178 at 2ug/ml. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.
Flow Cytometry: DIO2 Antibody [NBP1-00178]

Flow Cytometry: DIO2 Antibody [NBP1-00178]

Flow Cytometry: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody

Applications for DIO2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Frozen

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:128000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DIO2

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

5DII, D2, deiodinase, iodothyronine, type II, DIOII, EC 1.97.1, EC 1.97.1.10, ITDI2, SelY, TXDI2thyroxine deiodinase, type II, Type 2 DI, type 2 iodothyronine deiodinase, type II iodothyronine deiodinase, type-II 5'deiodinase, Type-II 5'-deiodinase

Entrez Gene IDs

1734 (Human); 13371 (Mouse); 65162 (Rat)

Gene Symbol

DIO2

UniProt

Q92813 (Human)

Additional DIO2 Products

Product Documents for DIO2 Antibody

Certificate of Analysis

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Product Specific Notices for DIO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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