Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Rabbit

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG
View all DIO3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]

Reactivity Notes

Rabbit reactivity via customer review.

Localization

Cell membrane; Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DIO3 Antibody [Biotin]

Flow Cytometry: DIO3 Antibody [Biotin] [NBP1-05767B] - Dio3 staining on mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.

Applications for DIO3 Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-05767B in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DIO3

Dio3, or type 3 iodothyronine deiodinase, can regulate circulating fetal thyroid hormone concentrations throughout gestation. It has an essential role for regulation of thyroid hormone inactivation during embryological development, and may play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Dio3 has been linked to the development of consumptive hypothyroidism in both infants and adults.

Alternate Names

5DIII, D3, deiodinase, iodothyronine, type III, DIOIII, EC 1.97.1, EC 1.97.1.11, ITDI3, placental type, thyroxine deiodinase type III (selenoprotein), Type 3 DI, type 3 iodothyronine selenodeiodinase, type III iodothyronine deiodinase, type-III 5' deiodinase, Type-III 5'-deiodinase

Gene Symbol

DIO3

Additional DIO3 Products

Product Documents for DIO3 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DIO3 Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DIO3 Antibody [Biotin]

Customer Reviews for DIO3 Antibody [Biotin] (1)

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1 Customer Rating
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Customer Images

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  • Name: Guanhua Xie
    Application: Flow Cytometry
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 09/03/2014
    Dio3 staining on mouse hepatic stellate cell cultured for 7 days
    DIO3 Antibody [Biotin] NBP1-05767B

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DIO3 Antibody [Biotin]

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  • Q: I am looking for a Dio3 peptide that I can inject in vivo to produce a biological effect. Can you provide information or confirm whether the Dio3 peptide you have would be effective as a biological agent in vivo?

    A: I have pulled up information on the Dio3 peptides and recombinant proteins that we have available. Unfortunately I would not expect any of them to be biologically active and capable of producing a response when injected in vivo. The two peptides we carry are simply intended to be used for negative control competition assays to block signal of the primary and determine what is specific and non-specific as far as signal is concerned. The other two recombinant proteins are ones we distribute for a Taiwanese company called Abnova and they should not be biologically active based on the cell free wheat germ system employed to synthesize them. We will typically list our active proteins with an indication of such on our datasheet if it has been tested and usually the application of functional listed.

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