Dishevelled-1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92847

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Dishevelled-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2). GQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dishevelled-1 Antibody - Azide and BSA Free

Dishevelled-1 Antibody - Azide and BSA Free

Western Blot: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] -

Western Blot: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Western blot analysis of various lysates, using Dishevelled-1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunohistochemistry-Paraffin: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847]

Immunohistochemistry-Paraffin: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847]

Immunohistochemistry-Paraffin: Dishevelled-1 Antibody [NBP2-92847] - Paraffin-embedded rat testis using Dishevelled-1.
Immunohistochemistry-Paraffin: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847]

Immunohistochemistry-Paraffin: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847]

Immunohistochemistry-Paraffin: Dishevelled-1 Antibody [NBP2-92847] - Paraffin-embedded mouse testis using Dishevelled-1.
Dishevelled-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] -

Immunocytochemistry/ Immunofluorescence: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Immunofluorescence analysis of C6 cells using Dishevelled-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Dishevelled-1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] -

Immunocytochemistry/ Immunofluorescence: Dishevelled-1 Antibody - Azide and BSA Free [NBP2-92847] - Immunofluorescence analysis of NIH-3T3 cells using Dishevelled-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Dishevelled-1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dishevelled-1

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq]

Alternate Names

Dishevelled1, Dsh, DVL1

Gene Symbol

DVL1

Additional Dishevelled-1 Products

Product Documents for Dishevelled-1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dishevelled-1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Wnt Family

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Protocols

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