DL-TBOA

Tocris Bioscience | Catalog # 1223

Selective non-transportable inhibitor of EAATs
Tocris Bioscience

Key Product Details

Description

Selective non-transportable inhibitor of EAATs
View all Glutamate (EAAT) Transporter Inhibitors »

Product Description

DL-TBOA is a competitive, non-transportable blocker of excitatory amino acid transporters (IC50 values are 6 μM, 6 μM and 70 μM, for EAAT2, EAAT3 and EAAT1, respectively). DL-TBOA also inhibits EAAT4 and EAAT5 (Ki values are 4.4 μM and 3.2 μM respectively). DL-TBOA displays high selectivity for EAATs over ionotropic and metabotropic glutamate receptors. In [3H]-d-Asp uptake assays in HEK293 cells expressing human EAAT2, EAAT1 and EAAT3, Ki values are 2.2, 2.9, and 9.3 μM, respectively. In a FLIPR Membrane Potential (FMP) assay, Km values are 0.59, 1.8 and 2.8 μM for human EAAT2, EAAT3 and EAAT1, respectively. DL-TBOA augments SN 38 (Cat. No. 2684) induced colorectal cancer cell death in drug-resistant cell lines. DL-TBOA enhances synaptic activity in the frog labyrinth.

Product Specifications for DL-TBOA

Molecular Weight

239.23

Formula

C11H13NO5

Storage

Desiccate at -20°C

Purity

≥98% (HPLC)

Chemical Name

DL-threo-β-Benzyloxyaspartic acid

CAS Number

205309-81-5

PubChem ID

90479771

InChI Key

CSEZCYBVEGIYNG-MFBWXLJUSA-N

SMILES

[H][C@@](OCC1=CC=CC=C1)(C(O)=O)[C@@]([H])(C(O)=O)N.[H][C@](OCC2=CC=CC=C2)(C(O)=O)[C@]([H])(C(O)=O)N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 1.2 5 with gentle warming
DMSO 23.92 100

Preparing Stock Solutions for DL-TBOA

The following data is based on the product molecular weight 239.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.18 mL 20.90 mL 41.80 mL
5 mM 0.84 mL 4.18 mL 8.36 mL
10 mM 0.42 mL 2.09 mL 4.18 mL
50 mM 0.08 mL 0.42 mL 0.84 mL

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Product Documents for DL-TBOA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DL-TBOA

For research use only

Customer Reviews for DL-TBOA (2)

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  • Neuron glia culture
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Mixed neuron-glia
    Verified Customer | Posted 07/12/2020
    Incubate for 24h
    TBOA (100 μM)
    DL-TBOA 1223
  • Slice Electrophysiology
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 04/09/2019
    Used at 100uM
    Used for slice electrophysiology experiments to inhibit glutamate transport.

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