DNA Polymerase beta Antibody - BSA Free

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• DNA Polymerase beta Antibody

  Name: Meiyi Tang

  Application: Slot blot DPC assay

  Sample Tested: Genomic DNA samples from LN428 cells -treated by copper complex to induce DNA-protein cross-links

  Species: Human

  Verified Customer | Posted 03/24/2017

  After the rabbit anti-DNA polymerase beta antibody from Novus Biologicals NB100-91734, which this lab has been using for DPC assay, being discontinued by Novus biologicals, we have been looking for an available antibody for the application of DPC assay. LN428 human cancer cells were treated by 100 uM of 1,10-Phenanthroline-Copper (II) complex to induce genomic DNA damages (DNA-protein cross-links); a serial of amount of gDNA samples (0, 200, 500, 1000 and 2000ng) were prepared and gDNA samples were blotted onto nitrocellulose membrane and probed for DNA polymerase beta which was trapped on the sites of gDNA damages. The NBP2-38600 antibody was compared with the discontinued DNA Polymerase beta antibody NB100-91734, which we have been using and has been working very well for DPC assay, The result showed that NBP2-38600 worked as good as, or evenbetter than, the discontinued NB100-91734. Slot blot DPC assay, a new and widely-used technique to detect DNA-Protein Cross-links (DPC) - induced by oxidative stresses in vitro (in tubes) or in vivo (in cells).

  

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