Dopamine beta-Hydroxylase Antibody

Novus Biologicals | Catalog # NBP1-31386

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dopamine beta-Hydroxylase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (86%), Bovine (80%).

Localization

Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen, Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen, secretory vesicle membrane, chromaffin granule membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopamine beta-Hydroxylase Antibody

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - RT2 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - U87 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Dopamine beta-Hydroxylase Antibody

Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] -

Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Dopamine beta-Hydroxylase Antibody

Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] -

Expression levels of monoamine-regulating enzymes in the brain of SMN delta 7 mice.a, h, o Representative autoradiograms of brain lysates immunoblots of SMN delta 7 and wild-type (WT) mice at a post-natal day 3, h day 6, and o day 11. b–g Protein levels quantification of b Tyrosine hydroxylase (TH), c phospho-Tyrosine hydroxylase at Ser-40 (P-Ser40-TH), d Dopamine beta hydroxylase (D beta H), e Tryptophan hydroxylase 2 (TPH2), f Aromatic amino acid decarboxylase (AADC) and g Monoamine Oxidase A (MAO-A) in SMN delta 7 (n = 4) and WT (n = 5) mice (except for TPH2: n = 4 mice/genotype) at post-natal day 3. i–n Protein levels quantification of i TH (n = 4 WT, n = 5 SMN delta 7), j P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), k D beta H (n = 4 WT, n = 4 SMN delta 7), l TPH2 (n = 4 WT, n = 3 SMN delta 7), m AADC (n = 4 WT, n = 5 SMN delta 7) and n MAO-A (n = 4 WT, n = 5 SMN delta 7) at post-natal day 6. p–u Protein levels quantification of p TH (n = 13 WT, n = 14 SMN delta 7), q P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), r D beta H (n = 4 WT, n = 5 SMN delta 7), s TPH2 (n = 4 WT, n = 5 SMN delta 7), t AADC (n = 13 WT, n = 14 SMN delta 7) and u MAO-A (n = 8 WT, n = 10 SMN delta 7) at post-natal day 11. Data are normalized to beta -Actin levels and shown as box and whisker plots representing the median with interquartile range (IQR). Dots represent individual mice values. *p < 0.05, **p < 0.01, compared with age-matched WT mice (unpaired t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37957344), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Dopamine beta-Hydroxylase Antibody

Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] -

Expression levels of monoamine-regulating enzymes in the brain of SMN delta 7 mice.a, h, o Representative autoradiograms of brain lysates immunoblots of SMN delta 7 and wild-type (WT) mice at a post-natal day 3, h day 6, and o day 11. b–g Protein levels quantification of b Tyrosine hydroxylase (TH), c phospho-Tyrosine hydroxylase at Ser-40 (P-Ser40-TH), d Dopamine beta hydroxylase (D beta H), e Tryptophan hydroxylase 2 (TPH2), f Aromatic amino acid decarboxylase (AADC) and g Monoamine Oxidase A (MAO-A) in SMN delta 7 (n = 4) and WT (n = 5) mice (except for TPH2: n = 4 mice/genotype) at post-natal day 3. i–n Protein levels quantification of i TH (n = 4 WT, n = 5 SMN delta 7), j P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), k D beta H (n = 4 WT, n = 4 SMN delta 7), l TPH2 (n = 4 WT, n = 3 SMN delta 7), m AADC (n = 4 WT, n = 5 SMN delta 7) and n MAO-A (n = 4 WT, n = 5 SMN delta 7) at post-natal day 6. p–u Protein levels quantification of p TH (n = 13 WT, n = 14 SMN delta 7), q P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), r D beta H (n = 4 WT, n = 5 SMN delta 7), s TPH2 (n = 4 WT, n = 5 SMN delta 7), t AADC (n = 13 WT, n = 14 SMN delta 7) and u MAO-A (n = 8 WT, n = 10 SMN delta 7) at post-natal day 11. Data are normalized to beta -Actin levels and shown as box and whisker plots representing the median with interquartile range (IQR). Dots represent individual mice values. *p < 0.05, **p < 0.01, compared with age-matched WT mice (unpaired t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37957344), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Dopamine beta-Hydroxylase Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dopamine beta-Hydroxylase

The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways. Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis. Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system. The disruption of the DBH gene is thought to cause changes in development and behavior in certain models. Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.

Alternate Names

DBH, DBM, Dopamine betaHydroxylase, DOPBHY

Entrez Gene IDs

1621 (Human)

Gene Symbol

DBH

UniProt

Additional Dopamine beta-Hydroxylase Products

Product Documents for Dopamine beta-Hydroxylase Antibody

Certificate of Analysis

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Product Specific Notices for Dopamine beta-Hydroxylase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Dopamine beta-Hydroxylase Antibody

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Protocols

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