Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25443

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1478 expressed in HEK293

Format

Azide and BSA Free
View all Dopamine D2 R/DRD2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-term region of Human Dopamine D2R/DRD2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%), Canine (84%), Porcine (88%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Dopamine Receptor D2 antibody [HL1478] detects Dopamine Receptor D2 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.

Applications for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Assay dependent

Functional Assay

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopamine D2 R/DRD2

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinson's disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

Long Name

Dopamine D2 Receptor

Alternate Names

D2DR, D2R, Dopamine D2R, DRD2

Gene Symbol

DRD2

Additional Dopamine D2 R/DRD2 Products

Product Documents for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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