Catalog Number: 3093
Alternate Names: Compound C, BML-275
Chemical Name: 6-[4-[2-(1-Piperidinyl)ethoxy]phenyl]-3-(4-pyridinyl)-pyrazolo[1,5-a]pyrimidine dihydrochloride
Biological Activity
Potent inhibitor of AMP-activated protein kinase (AMPK) (Ki = 109 nM). Displays no significant activity on several structurally related kinases including ZAPK, SYK, PKCθ, PKA and JAK3. Inhibits AMPK activation induced by AICAR (Cat. No. 2840) and metformin (Cat. No. 2864). Also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6). Promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro. Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in water and to 20 mM in DMSO with gentle warming
  • Purity:
  • Storage:
    Desiccate at RT
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Role of AMP-activated protein kinase in mechanism of metformin action.
    Zhou et al.
    J.Clin.Invest., 2001;108:1167
  2. Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism.
    Yu et al.
    Nat.Chem.Biol., 2008;4:33
  3. Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway.
    Vucicevic et al.
    Autophagy, 2011;7:40
  4. Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells.
    Hao et al.
    PLoS ONE, 2008;3:2904
  5. A reliable and efficient protocol for human pluripotent stem cell differentiation into the definitive endoderm based on dispersed single cells.
    Diekmann et al.
    Stem Cells Dev., 2015;24:190

The citations listed below are publications that use Tocris products. Selected citations for Dorsomorphin dihydrochloride include:

Showing Results 1 - 10 of 32

  1. Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analyses
    Authors: Kapeli Et al.
    Nature Communications
  2. Effects of thrombin on insulin signalling and glucose uptake in cultured human myotubes.
    Authors: Al-Bayati Et al.
    J Diabetes Complications
  3. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
    Authors: Mou Et al.
    Cell: Stem Cell
  4. Adenosine monophosphate-activated protein kinase activation mediates the leptin-induced attenuation of cognitive impairment in a streptozotocin-induced rat model.
    Authors: Zhu Et al.
  5. Rapid and robust generation of long-term self-renewing human neural stem cells with the ability to generate mature astroglia.
    Authors: Palm Et al.
    Int J Mol Sci
  6. Transcription factor binding dynamics during human ES cell differentiation.
    Authors: Tsankov Et al.
    Biomed Res Int
  7. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells.
    Authors: Liao Et al.
    Exp Ther Med
  8. Developmental regulation of tau splicing is disrupted in stem cell-derived neurons from frontotemporal dementia patients with the 10 + 16 splice-site mutation in MAPT.
    Authors: Sposito Et al.
    Sci Rep
  9. Comparative Study on the Cytoprotective Effects of Activated Protein C Treatment in Nonsteatotic and Steatotic Livers under Ischemia-Reperfusion Injury.
    Authors: Matsuda Et al.
    BMC Neurosci
  10. Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy.
    Authors: Khan Et al.
    J Pharmacol Exp Ther
  11. Chemical constituents analysis and antidiabetic activity validation of four fern species from Taiwan.
    Authors: Chen Et al.
    Exp Neurol
  12. The PRKAA1/AMPKα1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML.
    Authors: Obba Et al.
    Nat Protoc
  13. The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
    Authors: Huang Et al.
    Nat Genet
  14. Bmp suppression in mangrove killifish embryos causes a split in the body axis.
    Authors: Mourabit Et al.
    PLoS One
  15. BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.
    Authors: Graham Et al.
    Nat Neurosci
  16. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
    Authors: Huang Et al.
    Nat Biotechnol
  17. Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development.
    Authors: Holtzhausen Et al.
  18. Bmp and Shh signaling mediate the expression of satb2 in the pharyngeal arches.
    Authors: Sheehan-Rooney Et al.
    PLoS One
  19. Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
    Authors: Reinhardt Et al.
    PLoS One
  20. EGF stimulates Müller glial proliferation via a BMP-dependent mechanism.
    Authors: Ueki and Reh
  21. Moderate excess of pyruvate augments osteoclastogenesis.
    Authors: Fong Et al.
    Biol Open
  22. Epigallocatechin gallate (EGCG) stimulates autophagy in vascular endothelial cells: a potential role for reducing lipid accumulation.
    Authors: Kim Et al.
    J Biol Chem
  23. Distinct energy requirements for human memory CD4 T-cell homeostatic functions.
    Authors: Taub Et al.
    Stem Cells Dev
  24. Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.
    Authors: Pol Et al.
    Lab Chip
  25. Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
    Authors: Brafman Et al.
    Cell Metab
  26. Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments.
    Authors: Li Et al.
    Nat Commun
  27. Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma.
    Authors: Knelson Et al.
    J Clin Invest
  28. Liraglutide, a long-acting GLP-1 mimetic, and its metabolite attenuate inflammation after intracerebral hemorrhage.
    Authors: Hou Et al.
    J Cereb Blood Flow Metab
  29. Engineering artificial signaling centers to polarize embryoid body differentiation.
    Authors: Petersen Et al.
    Br J Pharmacol
  30. Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses.
    Authors: Shi Et al.
  31. 5-Aminoimidazole-4-carboxyamide-ribonucleoside (AICAR)-stimulated hepatic expression of Cyp4a10, Cyp4a14, Cyp4a31, and other peroxisome proliferator-activated receptor α-responsive mouse genes is AICAR 5'-monophosphate-dependent and AMP-activated
    Authors: Bumpus and Johnson
    Hum Mol Genet
  32. Orexin is required for brown adipose tissue development, differentiation, and function.
    Authors: Sellayah Et al.
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