Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11D7

Format

Azide and BSA Free
View all DPPIV/CD26 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free (NBP2-02154) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free

Western Blot: DPPIV/CD26 Antibody (OTI11D7)Azide and BSA Free [NBP2-70588]

Western Blot: DPPIV/CD26 Antibody (OTI11D7)Azide and BSA Free [NBP2-70588]

Western Blot: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD26 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD26.
Immunocytochemistry/ Immunofluorescence: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Immunocytochemistry/ Immunofluorescence: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD26.
Immunohistochemistry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Immunohistochemistry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Immunohistochemistry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588] - Lungs harvested from BALB/c mice at day 3. Dilution: 1:800
Flow Cytometry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Flow Cytometry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Flow Cytometry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588] - Analysis of Hela cells, using anti-CD26 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: DPPIV/CD26 Antibody (OTI11D7)Azide and BSA Free [NBP2-70588]

Western Blot: DPPIV/CD26 Antibody (OTI11D7)Azide and BSA Free [NBP2-70588]

Western Blot: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588] - Analysis of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells.
Immunohistochemistry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Immunohistochemistry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588]

Immunohistochemistry: DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free [NBP2-70588] - Staining of paraffin-embedded liver tissue using anti-CD26 mouse monoclonal antibody.

Applications for DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:500

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPPIV/CD26

CD26 is encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.

Long Name

Dipeptidyl-peptidase IV

Alternate Names

CD26, DPP4

Gene Symbol

DPP4

Additional DPPIV/CD26 Products

Product Documents for DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DPPIV/CD26 Antibody (OTI11D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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