Key Product Details

Species Reactivity

Multi-Species

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoaffinity Purification, Dot Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # J2

Format

Azide and BSA Free
View all dsRNA Primary Antibodies »

Product Specifications

Immunogen

Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.

Specificity

This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by this antibody, although in some assays its affinity to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for dsRNA Antibody (J2) - Azide and BSA Free

dsRNA Antibody (J2) - Azide and BSA Free

Immunohistochemistry-NBP3-11395-dsRNA Antibody (J2) - Azide and BSA Free-

Immunohistochemistry-NBP3-11395-dsRNA Antibody (J2) - Azide and BSA Free-Antibody J2 (1:250 dilution, 4 microgram/ml) reveals the colocalisation of dsRNA (labelled in green) and Hepatitis C virus core protein (labelled in red), indicating the location of putative virus assembly sites (arrows).
dsRNA Antibody (J2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: dsRNA Antibody (J2) - Azide and BSA Free [NBP3-11395] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
dsRNA Antibody (J2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: dsRNA Antibody (J2) - Azide and BSA Free [NBP3-11395] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for dsRNA Antibody (J2) - Azide and BSA Free

Application
Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry. The optimum working dilution of the antibody for any specific application should be established by titration.

Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.

Not for use for clinical purposes. For in vitro use only.

Immuno-affinity-chromatography.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

The lyophilised sample should be reconstituted with 200 ul (500 ul for 500 ug size) sterile distilled water. The mAb will then be in PBS without any stabilisers at a concentration of 1 mg/ml.

Formulation

Lyophilised from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: dsRNA

Double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. J2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.

Alternate Names

Double-stranded RNA

Additional dsRNA Products

Product Documents for dsRNA Antibody (J2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for dsRNA Antibody (J2) - Azide and BSA Free

After reconstitution antibodies should be aliquoted and stored at -20C or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for at least 5 years at -20C or -70C. This ds-RNA antibody was manufactured by Scicon.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for dsRNA Antibody (J2) - Azide and BSA Free

Customer Reviews for dsRNA Antibody (J2) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review dsRNA Antibody (J2) - Azide and BSA Free and earn rewards!

Have you used dsRNA Antibody (J2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies