DUOX2 Antibody - BSA Free

Novus Biologicals | Catalog # NB110-61576

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DUOX2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human DUOX2 (within residues 400-500). [Swiss-Prot# Q9NRD8]

Localization

Apical cell membrane; Multi-pass membrane protein. Note: Localizes to the apical membrane of epithelial cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DUOX2 Antibody - BSA Free

Knockdown Validated: DUOX2 Antibody - BSA Free [NB110-61576]

Western Blot: DUOX2 Antibody - BSA Free [NB110-61576]

DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg
Western Blot: DUOX2 AntibodyBSA Free [NB110-61576]

Western Blot: DUOX2 AntibodyBSA Free [NB110-61576]

Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.
Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody - BSA Free [NB110-61576]

Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody - BSA Free [NB110-61576]

Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: DUOX2 AntibodyBSA Free [NB110-61576]

Western Blot: DUOX2 AntibodyBSA Free [NB110-61576]

DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg
Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody - BSA Free [NB110-61576]

Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody - BSA Free [NB110-61576]

Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for DUOX2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24492313)

Western Blot

2 ug/ml
Application Notes
In Western blot a band is observed at ~150 kDa, and in Immunocytochemistry/Immunofluorescence membrane staining was seen in A431 cells.

Reviewed Applications

Read 2 reviews rated 2 using NB110-61576 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DUOX2

DUOX2 (dual oxidase 2), a multi-pass membrane protein localized to the apical membrane of epithelial cells, belongs to NADPH oxidase (NOX) family which is known to reduce molecular oxygen with NADPH as electron donor and unlike most of NOX-family members that produce superoxide anions (O2.-) as primary product, DUOX directly produce H2O2. DUOX2 and DUOX 1 require specific maturation factors, DUOXA1 and DUOXA2, to be functionally expressed at the apical follicular membrane, the site for hormonogenesis. DUOX2/DUOX 1 are major components of H2O2-generating system and generate H2O2 which is required for activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. DUOX2 plays a role in thyroid hormones synthesis and LPO-mediated antimicrobial defense mucosal surface. It has its own peroxidase activity through its N-terminal peroxidase-like domain which is inhibited by aminobenzohydrazide and the NADPH oxidase activity is calcium-dependent. DUOX2 is expressed in thyrocytes, intestine, kidney, liver, lung, pancreas, prostate, salivary glands, testis etc. and is induced by forskolin, thyrotropin and the Th1-specific cytokine IFNG/IFN-gamma. Defects in DUOX2 have been linked to thyroid dyshormonogenesis 6 (TDH6) characterized by defective conversion of accumulated iodide to organically bound iodine.

Alternate Names

dual oxidase 2, dual oxidase-like domains 2, EC 1.11.1.-, EC 1.6.3.1, flavoprotein NADPH oxidase, Large NOX 2, LNOX2P138(TOX), Long NOX 2, NADH/NADPH thyroid oxidase p138-tox, NADPH oxidase/peroxidase DUOX2, NADPH thyroid oxidase 2, nicotinamide adenine dinucleotide phosphate oxidase, NOXEF2, p138 thyroid oxidase, P138-TOX, THOX2TDH6, Thyroid oxidase 2

Gene Symbol

DUOX2

Additional DUOX2 Products

Product Documents for DUOX2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DUOX2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DUOX2 Antibody - BSA Free

Customer Reviews for DUOX2 Antibody - BSA Free (2)

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2 Customer Ratings
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Customer Images

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  • Protein expression of duox2
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    Protein expression of duox2 in mouse lung homogenates
    I tested the protein expression of duox2 in mouse lung homogenates treated with smoke and room air
    DUOX2 Antibody - BSA Free NB110-61576
  • Duox2 protein expression
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    duox2 protein expression using simple western JESS
    I checked for the protein expression of duox2 in mouse lung homogenate
    DUOX2 Antibody - BSA Free NB110-61576
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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Protocols

View specific protocols for DUOX2 Antibody - BSA Free (NB110-61576):

DUOX2 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

DUOX2 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 38 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-DUOX2 primary antibody (NB 110-61576) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DUOX2 Antibody - BSA Free

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  • Q: Can you please let me know the homology of the immunogen peptide with mouse, for NB110-61576? Here is a link to the mouse DUOX2 protein information: ncbi

    A: The homology is 84.6%.

  • Q: Hi, I am looking for an antibody to human DUOX2 that will work on human formalin fixed paraffin embedded sections. I notice from your website that you have several recommended for WB and wondered if you have any experience of their use in other areas?

    A: At this time we do not have an antibody for DUOX2 that works in IHC paraffin embedded sections. If you choose to try one in this application we have our Innovator's Reward program where we reward our customers for trying antibodies in previously untested applications and species.

  • Q: Can you please let me know the homology of the immunogen peptide with mouse, for NB110-61576? Here is a link to the mouse DUOX2 protein information: ncbi

    A: The homology is 84.6%.

  • Q: Hi, I am looking for an antibody to human DUOX2 that will work on human formalin fixed paraffin embedded sections. I notice from your website that you have several recommended for WB and wondered if you have any experience of their use in other areas?

    A: At this time we do not have an antibody for DUOX2 that works in IHC paraffin embedded sections. If you choose to try one in this application we have our Innovator's Reward program where we reward our customers for trying antibodies in previously untested applications and species.

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