DUX4 Antibody (P4H2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80703
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P4H2
Format
Azide and BSA Free
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Product Specifications
Immunogen
Synthetic peptide corresponding to the C-terminus of human DUX4. [Swiss-Prot: Q9UBX2]
Localization
Nucleus. Note: Actively transported through the nuclear pore complex (NPC)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DUX4 Antibody (P4H2) - Azide and BSA Free
Western Blot: DUX4 Antibody (P4H2)Azide and BSA Free [NBP2-80703]
Western Blot: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Total protein from human testis tissue and human K562, HeLa and HepG2 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-DUX4 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703]
Immunocytochemistry/Immunofluorescence: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Detection of human DUX4 on C2C12 mouse myoblasts transfected with pCS2-DUX4. Cells were counterstained with DAPI for nuclei. Image from the standard format of this antibody.Immunohistochemistry: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703]
Immunohistochemistry: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Analysis of DUX4 on human testis Image from the standard format of this antibody.Western Blot: DUX4 Antibody (P4H2)Azide and BSA Free [NBP2-80703]
Western Blot: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Analysis of DUX4 expression in (1) human testis and (2) DUX4 protein lysates. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703]
Immunocytochemistry/Immunofluorescence: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - A small number of FSHD muscle cells express a relatively large amount of DUX4. Cultured FSHD muscle cells were differentiated and immunostained with mAbs to DUX4. Cells were co-stained with the E5-5 rabbit mAb to the DUX4 C-terminal region (panels a, c, e) and the P2G4 mouse mAb to the N-term. region (panels b,d,f), or co-stained with the P4H2 mouse mAb to the C-terminal region and the E14-3 rabbit mAb to the N-term. region. Approximately 1 cell per 1000 showed nuclear staining and the co-localization of both the N-term. and C-term. regions indicates that these cells are expressing the full-length DUX4 protein. No positive nuclei were apparent in the control muscle cultures. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1001181), licensed under a CC-BY license. Image from the standard format of this antibody.Simple Western: DUX4 Antibody (P4H2)Azide and BSA Free [NBP2-80703]
Simple Western: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Lane view shows a specific band for DUX4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.Applications for DUX4 Antibody (P4H2) - Azide and BSA Free
Application
Recommended Usage
ELISA
reported in scientific literature (PMID 21529284)
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:100-1:200
Immunohistochemistry-Frozen
1:100-1:200
Immunohistochemistry-Paraffin
1:100-1:200
Simple Western
1:10
Western Blot
0.5-2.0ug/ml
Application Notes
In Western blot, a band can be seen at approx. 52 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:10. Separated by Size-Wes, Sally Sue/Peggy Sue.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:10. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DUX4
Long Name
Double Homeobox 4
Alternate Names
DUX10, DUX4L1
Gene Symbol
DUX4
Additional DUX4 Products
Product Documents for DUX4 Antibody (P4H2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DUX4 Antibody (P4H2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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