E-Cadherin Antibody (4A2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-54587
![Western Blot: E-Cadherin Antibody (4A2)Azide and BSA Free [NBP2-54587]](https://resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Azide-and-BSA-Free-Western-Blot-NBP2-54587-img0006.jpg "Western Blot: E-Cadherin Antibody (4A2)Azide and BSA Free [NBP2-54587]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4A2
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human E-Cadherin protein (Uniprot: P12830 )
Localization
Cell Surface
Marker
Intercellular Junction Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53229).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for E-Cadherin Antibody (4A2) - Azide and BSA Free
Western Blot: E-Cadherin Antibody (4A2)Azide and BSA Free [NBP2-54587]
Western Blot: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]](https://resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54587-img0007.jpg "Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]")
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]
Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.![Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]](https://resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54587-img0001.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (4A2).![Flow Cytometry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]](https://resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54587-img0004.jpg "Flow Cytometry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]")
Flow Cytometry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]
Flow Cytometry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Flow Cytometric Analysis of human trypsinized MCF-7 cells using E-Cadherin Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).![Immunohistochemistry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]](https://resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-54587-img0003.jpg "Immunohistochemistry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]")
Immunohistochemistry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587]
Immunohistochemistry: E-Cadherin Antibody (4A2) - Azide and BSA Free [NBP2-54587] - Staining of human pancreatic tumor. Image from a verified customer review.Applications for E-Cadherin Antibody (4A2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-54587 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (4A2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for E-Cadherin Antibody (4A2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for E-Cadherin Antibody (4A2) - Azide and BSA Free (1)
5 out of 5
1 Customer Rating
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Application: Imaging Mass CytometrySample Tested: Pancreatic tumorSpecies: HumanVerified Customer | Posted 09/25/2018Heat mediated antigen retrieval performed in citrate buffer for 30 mins at 95C.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
