E-Cadherin Antibody (4A2)
Novus Biologicals | Catalog # NBP2-53229
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4A2
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Product Specifications
Immunogen
Recombinant human E-Cadherin protein (Uniprot: P12830 )
Localization
Cell Surface
Marker
Intercellular Junction Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54587)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for E-Cadherin Antibody (4A2)
![Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Western-Blot-NBP2-53229-img0005.jpg "Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229]")
Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229]
Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Immunocytochemistry-Immunofluorescence-NBP2-53229-img0007.jpg "Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229]")
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229]
Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229] - Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.![Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Immunohistochemistry-NBP2-53229-img0003.jpg "Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229]")
Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229]
Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229] - Staining of human pancreatic tumor. Image from a verified customer review.![Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Flow-Cytometry-NBP2-53229-img0006.jpg "Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229]")
Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229]
Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229] - Flow Cytometric Analysis of human trypsinized MCF-7 cells using E-Cadherin Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).![Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229]](https://beta-resources.rndsystems.com/images/products/E-Cadherin-Antibody-4A2-Immunohistochemistry-Paraffin-NBP2-53229-img0001.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229]")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229]
Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (4A2).Applications for E-Cadherin Antibody (4A2)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
4 ug/mL
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
UniProt
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (4A2)
Product Specific Notices for E-Cadherin Antibody (4A2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
