E-Cadherin Antibody (CL1170) - BSA Free

Novus Biologicals | Catalog # NBP2-34475

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL1170

Format

BSA Free
View all E-Cadherin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P12830, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Rat (80%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for E-Cadherin Antibody (CL1170) - BSA Free

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.
Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]

Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475]

Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human prostate shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human colorectal cancer shows moderate to strong membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.

Applications for E-Cadherin Antibody (CL1170) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (CL1170) - BSA Free

Certificate of Analysis

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Product Specific Notices for E-Cadherin Antibody (CL1170) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail