E-Cadherin Antibody (DECMA-1)

Novus Biologicals | Catalog # NB120-11512

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Feline

Cited:

Mouse, Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # DECMA-1
View all E-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse embryonal carcinoma cell line PCC4 Aza RI.

Localization

Cell membrane; cell-cell junction; single-pass type I membrane protein

Marker

Epithelial Cell Marker, Adherens Junctions Marker

Specificity

Antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for E-Cadherin Antibody (DECMA-1)

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Adult mouse small intestine stained with NB12-11512. Image from verified customer review.
Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1. Detection using Goat Anti-Rat IgG, FITC conjugate.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 diluted to 1:3200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.

Applications for E-Cadherin Antibody (DECMA-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1600

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:3000
Application Notes
The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in westernblot or immunohistochemical assays.

Reviewed Applications

Read 2 reviews rated 4 using NB120-11512 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Raw ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 282637 (Bovine)

Gene Symbol

CDH1

UniProt

P09803

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (DECMA-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for E-Cadherin Antibody (DECMA-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for E-Cadherin Antibody (DECMA-1)

Customer Reviews for E-Cadherin Antibody (DECMA-1) (2)

4 out of 5
2 Customer Ratings
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1 Stars
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Customer Images

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  • E-Cadherin Antibody (DECMA-1)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 06/06/2019
    small intestine stained with NB120-11512
    E-Cadherin Antibody (DECMA-1) NB120-11512
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: cat testis, cat ovary, HEK293 whole cell lysate
    Species: Other
    Verified Customer | Posted 11/18/2013
    E-Cadherin Antibody (DECMA-1) NB120-11512

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail