E-Cadherin Antibody

Novus Biologicals | Catalog # NBP2-16258

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Zebrafish

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all E-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Reactivity Notes

Bovine (82%), Pig (83%).

Localization

Cell junction; Cell membrane

Marker

Epithelial Cell Marker, Adherens Junctions Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit E-Cadherin Antibody (NBP2-16258) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-E-Cadherin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for E-Cadherin Antibody

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse stomach. E-Cadherin stained by E-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: E-Cadherin Antibody [NBP2-16258]

Western Blot: E-Cadherin Antibody [NBP2-16258]

Western Blot: E-Cadherin Antibody [NBP2-16258] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody.
Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse cervix. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Rat intestine. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Breast cancer. E-cadherin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Rat prostate. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258]

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse intestine. E-Cadherin stained by E-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: E-Cadherin Antibody [NBP2-16258]

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258]

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258] - MCF-7 whole cell extract A. Control with 3 ug of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 ug E-cadherin antibody 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Immunoprecipitation: E-Cadherin Antibody [NBP2-16258]

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258]

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258] - Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 ug of E-Cadherin antibody. Western blot analysis was performed using E-Cadherin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
E-Cadherin Antibody

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin Antibody

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (NBP2-16258) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin Antibody

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] -

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
E-Cadherin Antibody

Western Blot: E-Cadherin Antibody [NBP2-16258] -

Western Blot: E-Cadherin Antibody [NBP2-16258] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
E-Cadherin Antibody

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] -

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin Antibody

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] -

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.
E-Cadherin Antibody

Western Blot: E-Cadherin Antibody [NBP2-16258] -

Western Blot: E-Cadherin Antibody [NBP2-16258] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
E-Cadherin Antibody

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] -

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.
E-Cadherin Antibody

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] -

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.
Blue: Hoechst 33342 staining.
E-Cadherin Antibody

Western Blot: E-Cadherin Antibody [NBP2-16258] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
E-Cadherin Antibody

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] -

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
E-Cadherin antibody (NBP2-16258) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin Antibody

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] -

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for E-Cadherin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Proximity Ligation Assay

Assay dependent

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: E-Cadherin

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody

Certificate of Analysis

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Product Specific Notices for E-Cadherin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for E-Cadherin Antibody

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail