eEF-2 Antibody (4E2Z5)

Novus Biologicals | Catalog # NBP3-16866

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4E2Z5 expressed in HEK293
View all eEF-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF-2 (P13639). IYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eEF-2 Antibody (4E2Z5)

Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of various cell lines, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of Rat spleen, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Immunohistochemistry-Paraffin: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Immunohistochemistry-Paraffin: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunohistochemistry of paraffin-embedded human appendix using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866]

Immunocytochemistry/Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of NIH-3T3 cells using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
eEF-2 Antibody (4E2Z5)

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] -

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of C6 cells using eEF-2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
eEF-2 Antibody (4E2Z5)

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] -

Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of U-2 OS cells using eEF-2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for eEF-2 Antibody (4E2Z5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eEF-2

The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Long Name

Eukaryotic Translation Elongation Factor 2

Alternate Names

eEF2

Gene Symbol

EEF2

Additional eEF-2 Products

Product Documents for eEF-2 Antibody (4E2Z5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for eEF-2 Antibody (4E2Z5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

mTOR Signaling Pathway mTOR Signaling Pathway Thumbnail