EEF1D Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38001

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EEF1D Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).

Sequence:
MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEF1D Antibody - BSA Free

EEF1D Antibody

Western Blot: EEF1D Antibody [NBP3-38001] -

Western Blot: EEF1D Antibody [NBP3-38001] - Western blot analysis of various lysates using EEF1D Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
EEF1D Antibody

Immunoprecipitation: EEF1D Antibody [NBP3-38001] -

Immunoprecipitation: EEF1D Antibody [NBP3-38001] - Immunoprecipitation of EEF1D in 300 ug extracts from Jurkat cells using 3 ug EEF1D Rabbit pAb (A2509). Western blot analysis was performed using EEF1D Rabbit pAb (A2509) at 1:500 dilution.
EEF1D Antibody

Immunohistochemistry: EEF1D Antibody [NBP3-38001] -

Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1D Antibody

Immunohistochemistry: EEF1D Antibody [NBP3-38001] -

Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1D Antibody

Immunocytochemistry/ Immunofluorescence: EEF1D Antibody [NBP3-38001] -

Immunocytochemistry/ Immunofluorescence: EEF1D Antibody [NBP3-38001] - Immunofluorescence analysis of PC-12 cells using EEF1D Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
EEF1D Antibody

Immunoprecipitation: EEF1D Antibody [NBP3-38001] -

Immunoprecipitation: EEF1D Antibody [NBP3-38001] - Immunoprecipitation of EEF1D in 300 ug extracts from jurkat cells using 3 ug EEF1D Rabbit pAb at 1:500 dilution.
EEF1D Antibody

Immunohistochemistry: EEF1D Antibody [NBP3-38001] -

Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1D Antibody

Immunohistochemistry: EEF1D Antibody [NBP3-38001] -

Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
EEF1D Antibody

Immunocytochemistry/ Immunofluorescence: EEF1D Antibody [NBP3-38001] -

Immunocytochemistry/ Immunofluorescence: EEF1D Antibody [NBP3-38001] - Immunofluorescence analysis of HeLa cells using EEF1D Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for EEF1D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EEF1D

EEF1D encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Antigen NY-CO-4, EF-1D, EF-1-delta, EF1DFP1047, elongation factor 1-delta, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein), FLJ20897, guanine nucleotide exchange protein

Gene Symbol

EEF1D

Additional EEF1D Products

Product Documents for EEF1D Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EEF1D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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