EEF1E1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32184

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EEF1E1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEF1E1 Antibody - BSA Free

Western Blot: EEF1E1 Antibody [NBP1-32184]

Western Blot: EEF1E1 Antibody [NBP1-32184]

Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EEF1E1 Antibody [NBP1-32184]

Immunocytochemistry/ Immunofluorescence: EEF1E1 Antibody [NBP1-32184]

Immunocytochemistry/Immunofluorescence: EEF1E1 Antibody [NBP1-32184] - Analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184]

Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184]

Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: EEF1E1 Antibody [NBP1-32184]

Western Blot: EEF1E1 Antibody [NBP1-32184]

Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184]

Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184]

Immunohistochemistry-Paraffin: EEF1E1 Antibody [NBP1-32184] - Mouse fore brain. EEF1E1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Knockout Validated: EEF1E1 Antibody [NBP1-32184]

Western Blot: EEF1E1 Antibody [NBP1-32184]

Western Blot: EEF1E1 Antibody [NBP1-32184] - Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for EEF1E1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EEF1E1

This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, ARS-interacting multifunctional protein 3, Elongation factor p18, eukaryotic translation elongation factor 1 epsilon 1, eukaryotic translation elongation factor 1 epsilon-1, Multisynthase complex auxiliary component p18, P18, p18 component of aminoacyl-tRNA synthetase complex

Gene Symbol

EEF1E1

Additional EEF1E1 Products

Product Documents for EEF1E1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EEF1E1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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