EFHD2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31134

Novus Biologicals
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Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EFHD2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EFHD2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EFHD2 Antibody - BSA Free

Western Blot: EFHD2 Antibody [NBP1-31134]

Western Blot: EFHD2 Antibody [NBP1-31134]

Western Blot: EFHD2 Antibody [NBP1-31134] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (NBP1-31134). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: EFHD2 Antibody [NBP1-31134]

Western Blot: EFHD2 Antibody [NBP1-31134]

Western Blot: EFHD2 Antibody [NBP1-31134] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody diluted at 1:1000.
EFHD2 Antibody

Western Blot: EFHD2 Antibody [NBP1-31134] -

Western Blot: EFHD2 Antibody [NBP1-31134] - Sample(30 ug of whole cell lysate)
A:H1299
10% SDS PAGE
NBP1-31134 diluted at 1:1000
EFHD2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EFHD2 Antibody [NBP1-31134] -

EFHD2 antibody detects EFHD2 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EFHD2 protein stained by EFHD2 antibody (NBP1-31134) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
EFHD2 Antibody - BSA Free

Immunohistochemistry-Frozen: EFHD2 Antibody [NBP1-31134] -

EFHD2 antibody detects EFHD2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: EFHD2 protein stained by EFHD2 antibody (NBP1-31134) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for EFHD2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EFHD2

Found in lymphocytes; preferentially expressed in CD8+ cells. Contains 2 EF Hand domains.

Alternate Names

EF hand domain containing 2, EF hand domain family, member D2, EF-hand domain family, member D2, EF-hand domain-containing protein D2, MGC4342, swiprosin 1, swiprosin-1, SWS1

Gene Symbol

EFHD2

Additional EFHD2 Products

Product Documents for EFHD2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EFHD2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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