EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80715

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EG188e/d5

Format

Azide and BSA Free
View all EGLN3/PHD3 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human PHD3/HIF Prolyl Hydroxylase 3 (UniProt# Q9H6Z9)

Localization

Cytoplasm. Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)Azide and BSA Free [NBP2-80715]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)Azide and BSA Free [NBP2-80715]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free [NBP2-80715] - PHD3 expression in SK-N-BE(2) whole cell lysates. Cells were treated in normoxia (left) and in hypoxia (right). Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free [NBP2-80715]

Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free [NBP2-80715]

Immunohistochemistry: EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free [NBP2-80715] - Analysis of HIF Prolyl Hydroxylase 3 in human renal cancer using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)Azide and BSA Free [NBP2-80715]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5)Azide and BSA Free [NBP2-80715]

Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free [NBP2-80715] - Analysis of HIF Prolyl Hydroxylase 3 in (1) Cobalt chloride treated COS-7 nuclear extracts and (2) Untreated COS-7 nuclear extracts. Image from the standard format of this antibody.
Simple Western: EGLN3/PHD3 Antibody (EG188e/d5)Azide and BSA Free [NBP2-80715]

Simple Western: EGLN3/PHD3 Antibody (EG188e/d5)Azide and BSA Free [NBP2-80715]

Simple Western: EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free [NBP2-80715] - Lane view shows a specific band for PHD3/HIF Prolyl Hydroxylase 3 in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Simple Western

1:1000

Western Blot

10 ug/ml
Application Notes

This HIF Prolyl Hydroxylase 3 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen approx. 27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:1000. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGLN3/PHD3

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitination complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in DNA damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.

Long Name

Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3

Alternate Names

HIFPH3, PHD3

Gene Symbol

EGLN3

Additional EGLN3/PHD3 Products

Product Documents for EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EGLN3/PHD3 Antibody (EG188e/d5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

HIF Enhancer Pathways HIF Enhancer Pathway Thumbnail