EHD2 Antibody

Novus Biologicals | Catalog # NB100-1458

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EHD2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CRLVPPSKRRHKGSA corresponding to C-Terminus according to NP_055416.2.

Localization

Nuclear

Specificity

This antibody is expected to recognise EHD1 protein as well as EHD2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EHD2 Antibody

EHD2 Antibody

Western Blot: Goat Polyclonal EHD2 Antibody [NB100-1458] -

Western Blot: Goat Polyclonal EHD2 Antibody [NB100-1458] - (0.1g/ml) staining of Human Lung (A), Colon (B) and Placenta (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: EHD2 Antibody [NB100-1458]

Immunocytochemistry/ Immunofluorescence: EHD2 Antibody [NB100-1458]

Immunocytochemistry/Immunofluorescence: EHD2 Antibody [NB100-1458] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: EHD2 Antibody [NB100-1458]

Immunocytochemistry/ Immunofluorescence: EHD2 Antibody [NB100-1458]

Immunocytochemistry/Immunofluorescence: EHD2 Antibody [NB100-1458] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: EHD2 Antibody [NB100-1458]

Flow Cytometry: EHD2 Antibody [NB100-1458]

Flow Cytometry: EHD2 Antibody [NB100-1458] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for EHD2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Approx 70-75kDa band observed in Human Lung, Colon and Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EHD2

EHD2 is homologous to human EHD1. The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus. Northern blot analysis detected a EHD2 transcript that is highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle, as well as different transcript that is expressed at low levels in heart and skeletal muscle.

Alternate Names

EH domain containing 2, EH domain-containing protein 2, EH-domain containing 2, FLJ96617, PAST homolog 2, PAST2

Entrez Gene IDs

30846 (Human)

Gene Symbol

EHD2

UniProt

NP_055416. (Human)

Additional EHD2 Products

Product Documents for EHD2 Antibody

Certificate of Analysis

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Product Specific Notices for EHD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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