eIF2A [p Ser51] Antibody (SZ01-06)

Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353]

Western blot analysis on different lysates with NBP2-67353 at 1/2,000 dilution. Lane 1: HeLa whole cell lysate (15 µg/Lane) Lane 2: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate (15 µg/Lane) Lane 3: NIH/3T3 whole cell lysate (15 µg/Lane) Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (15 µg/Lane) Lane 5: C6 whole cell lysate (20 µg/Lane) Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: Lane 1-4: 2 minutes; Lane 5-6: 23 seconds； 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67353 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.