eIF2A [p Ser51] Antibody

Novus Biologicals | Catalog # NB100-81896

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine, Fish

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all eIF2A Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human eIF2A around the phosphorylation site of serine 51 (E-L-SP-R-R).

Modification

p Ser51

Specificity

Detects endogenous levels of eIF2A only when phosphorylated at serine 51.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2A [p Ser51] Antibody

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896]

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896]

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896]

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896]

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.
eIF2A [p Ser51] Antibody

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] -

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,
eIF2A [p Ser51] Antibody

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] -

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).
eIF2A [p Ser51] Antibody

Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] -

Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896

Applications for eIF2A [p Ser51] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-200

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2A

eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, EIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Entrez Gene IDs

83939 (Human); 229317 (Mouse); 502531 (Rat)

Gene Symbol

EIF2A

UniProt

P05198

Additional eIF2A Products

Product Documents for eIF2A [p Ser51] Antibody

Certificate of Analysis

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Product Specific Notices for eIF2A [p Ser51] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for eIF2A [p Ser51] Antibody

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Protocols

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