eIF2B epsilon Antibody (5M2D4)

Novus Biologicals | Catalog # NBP3-16674

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5M2D4 expressed in HEK293
View all eIF2B epsilon Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2B epsilon (Q13144). MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2B epsilon Antibody (5M2D4)

Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]

Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]

Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
eIF2B epsilon Antibody (5M2D4)

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
eIF2B epsilon Antibody (5M2D4)

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
eIF2B epsilon Antibody (5M2D4)

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human colon tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
eIF2B epsilon Antibody (5M2D4)

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast cancer tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
eIF2B epsilon Antibody (5M2D4)

Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -

Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Confocal imaging of MCF7 cells using eIF2B epsilon Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for eIF2B epsilon Antibody (5M2D4)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF2B epsilon

The eIF2B epsilon gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other E

Alternate Names

CACH, CLE, EIF-2B, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2BE, EIF2Bepsilon, eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD), eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, LVWM, translation initiation factor eIF-2B subunit epsilon

Gene Symbol

EIF2B5

Additional eIF2B epsilon Products

Product Documents for eIF2B epsilon Antibody (5M2D4)

Certificate of Analysis

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Product Specific Notices for eIF2B epsilon Antibody (5M2D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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