eIF4B Antibody

Novus Biologicals | Catalog # NBP2-16306

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Rat (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all eIF4B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human EIF4B. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4B Antibody

Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000
Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-16306]

Immunocytochemistry/ Immunofluorescence: eIF4B Antibody [NBP2-16306]

Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-16306] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306]

Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306]

Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306] - Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.
Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.
Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306]

Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000

Applications for eIF4B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF4B

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Long Name

Eukaryotic Translation Initiation Factor 4B

Alternate Names

Eif4a2

Gene Symbol

EIF4B

UniProt

P23588

Additional eIF4B Products

Product Documents for eIF4B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for eIF4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Translation Regulation

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Protocols

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FAQs

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