eIF4G1 Antibody (2A9) - Azide and BSA Free

Novus Biologicals | Catalog # H00001981-M10

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2A9

Format

Azide and BSA Free
View all eIF4G1 Primary Antibodies »

Product Specifications

Immunogen

EIF4G1 (NP_886553, 1500 a.a. ~ 1599 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

EIF4G1 (2A9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for eIF4G1 Antibody (2A9) - Azide and BSA Free

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody (M10), clone 2A9. Lane 1: EIF4G1 transfected lysatE (70.95 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: eIF4G1 Antibody (2A9) [H00001981-M10]

Immunocytochemistry/ Immunofluorescence: eIF4G1 Antibody (2A9) [H00001981-M10]

Immunocytochemistry/Immunofluorescence: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of monoclonal antibody to EIF4G1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in PC-12 (Cat # L012V1).
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in HepG2 (Cat # L019V1).
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10]

Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in NIH/3T3 (Cat # L018V1).
ELISA: eIF4G1 Antibody (2A9) [H00001981-M10]

ELISA: eIF4G1 Antibody (2A9) [H00001981-M10]

ELISA: eIF4G1 Antibody (2A9) [H00001981-M10] - Detection limit for recombinant GST tagged EIF4G1 is approximately 0.03ng/ml as a capture antibody.

Applications for eIF4G1 Antibody (2A9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF4G1

The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.

Long Name

Eukaryotic Translation Initiation Factor 4 gamma 1

Alternate Names

DKFZp686A1451, EIF4F, eIF-4G 1, eIF-4G1, EIF4-gamma, eIF-4-gamma 1, EIF4GI, EIF4Gp220, eucaryotic translation initiation factor 4G, eukaryotic translation initiation factor 4 gamma 1, eukaryotic translation initiation factor 4 gamma, 1, P220

Entrez Gene IDs

1981 (Human)

Gene Symbol

EIF4G1

OMIM

600495 (Human)

UniProt

NP_886553 (Human)

Additional eIF4G1 Products

Product Documents for eIF4G1 Antibody (2A9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for eIF4G1 Antibody (2A9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Translation Regulation

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Protocols

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FAQs

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