ELAVL2 Antibody (CL15323) - BSA Free
Novus Biologicals | Catalog # NBP3-43661
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # CL15323
Format
BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of Q12926, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for ELAVL2 Antibody (CL15323) - BSA Free
![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-241202515454919.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-24120251545878.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of human cerebellum shows strong cytoplasmic positivity in a subset of neurons in the granular layer.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-241202515454960.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-24120251546454.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of rat hippocampus shows strong cytoplasmic positivity in the dentate gyrus neurons.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-24120251545843.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-241202515454927.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of rat cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.![ELAVL2 Antibody (CL15323) [Unconjugated] Western blot: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-241202515454982.jpg "Western blot: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Western blot: ELAVL2 Antibody (CL15323) [NBP3-43661]
Analysis in human cell line SCLC-21H.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-2412025154585.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-24120251545858.jpg "Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunohistochemistry-Paraffin: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.![ELAVL2 Antibody (CL15323) [Unconjugated] Immunocytochemistry/ Immunofluorescence : ELAVL2 Antibody (CL15323) [NBP3-43661]](https://resources.rndsystems.com/images/products/nbp3-43661_mouse-elavl2-mab-cl15323-241202515532510.jpg "Immunocytochemistry/ Immunofluorescence: ELAVL2 Antibody (CL15323) [NBP3-43661]")
Immunocytochemistry/ Immunofluorescence: ELAVL2 Antibody (CL15323) [NBP3-43661]
Staining in A-431 cell line with ELAVL2 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).Applications for ELAVL2 Antibody (CL15323) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000-1:2500
Immunohistochemistry-Paraffin
1:1000-1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ELAVL2
Alternate Names
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B), ELAV-like neuronal protein 1, ELAV-like protein 2, HELN1, HEL-N1, Hu-antigen B, HuB, HUBELAV (embryonic lethal, abnormal vision, Drosophila)-like 2, Nervous system-specific RNA-binding protein Hel-N1
Gene Symbol
ELAVL2
Additional ELAVL2 Products
Product Documents for ELAVL2 Antibody (CL15323) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ELAVL2 Antibody (CL15323) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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