Endoglin/CD105 Antibody (SN6) - BSA Free

Novus Biologicals | Catalog # NB100-65601

Clone SN6 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SN6

Format

BSA Free
View all Endoglin/CD105 Primary Antibodies »

Product Specifications

Immunogen

Partially purified cell membrane antigens from fresh leukemia cells

Reactivity Notes

Predicted cross-reactivities: Cynomolgus monkey, Equine, Primate, Rhesus Monkey

Marker

Neo-endothelial Cells Marker

Specificity

Recognizes the human CD105 cell surface antigen, a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukemia cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Endoglin/CD105 Antibody (SN6) - BSA Free

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105RPE
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Analysis using the FITC conjugate of NB100-65601. Staining of KG1 cells.
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (SN6) NB100-65601PCP (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.

Applications for Endoglin/CD105 Antibody (SN6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Alternate Names

CD105, ENG

Gene Symbol

ENG

UniProt

P17813

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (SN6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Endoglin/CD105 Antibody (SN6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Endoglin/CD105 Antibody (SN6) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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