Endophilin B1/Bif-1 Antibody

Novus Biologicals | Catalog # NB100-820

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Endophilin B1/Bif-1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QKGKVPITYLELLN corresponding to C-Terminus according to NP_057093.1.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Endophilin B1/Bif-1 Antibody

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820]

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820]

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of NIH3T3 (A), RAW264.7 (B) and KNRK (C) cell lysate with antibody at 0.1 ug/mL. RIPA buffer, 35 ug total protein per lane. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820]

Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820]

Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820]

Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820]

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820]

Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of HEK293 (A) and A431 (B) cell lysate with antibody at 0.1 ug/mL. RIPA buffer, 35 ug total protein per lane. Detected by chemiluminescence.

Applications for Endophilin B1/Bif-1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 38 kDa band observed in lysates of human T-cell line Jurkat and in human lung and placenta (calculated MW of 40.8 kDa band according to NP_057093.1). A minor band of unknown identity was also consistently observed at 50 kDa band in Jurkat and placenta. This band was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endophilin B1/Bif-1

Bif-1 is an evolutionarily conserved cytoplasmic protein that contains a predicted Src homology 3 (SH3) domain located near its C terminus but shares no significant homology with members of the Bcl-2 family. A Northern blot analysis indicates that Bif-1 is expressed in most tissues with abundant expression in heart and skeletal muscle. Bif-1 is capable of interacting with Bax as demonstrated by yeast two-hybrid, coimmunoprecipitation, and immunofluorescence studies. Induction of apoptosis in murine pre-B hematopoietic cells FL5.12 by interleukin-3 withdrawal results in increased association of Bax with Bif-1, which is accompanied by a conformational change in the Bax protein. Overexpression of Bif-1 promotes Bax conformational change, caspase activation, and apoptotic cell death in FL5.12 cells following interleukin-3 deprivation.

Long Name

SH3-domain GRB2-like Endophilin B1/Bax-interacting Factor 1

Alternate Names

Bif-1, Bif1, SH3GLB1

Entrez Gene IDs

51100 (Human); 54673 (Mouse)

Gene Symbol

SH3GLB1

UniProt

NP_057093 (Human)

Additional Endophilin B1/Bif-1 Products

Product Documents for Endophilin B1/Bif-1 Antibody

Certificate of Analysis

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Product Specific Notices for Endophilin B1/Bif-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Endophilin B1/Bif-1 Antibody

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Protocols

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