Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free

Novus Biologicals | Catalog # NBP1-05170

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunoaffinity Purification

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A76

Format

BSA Free
View all Endorepellin/Perlecan Primary Antibodies »

Product Specifications

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix

Specificity

Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170]

Immunocytochemistry/ Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170]

Endorepellin-Perlecan-Heparan-Sulfate-Proteoglycan-Antibody-A76-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05170-img0001.jpg
Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] -

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] - Human primary brain microvascular endothelial cells stained for PDGF-BB (NBP1-58279), red and HSPG (NBP1-05170), green and nuclear DAPI blue. Image from a verified customer review.
Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free

Immunocytochemistry/ Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] -

hS/PCs sequentially secrete basement membrane proteins during microstructure reorganization and function. Encapsulated hS/PCs in HA-PEGDA hydrogels initially produce laminin (red) (A–C) and collagen IV (green) (D–F) even at the single-cell state (A,D). Perlecan (green) (G–I) secretion follows to stabilize the laminin and collagen IV networks. Scalebar = 20 μm in all confocal micrographs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31750298), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications

Application
Recommended Usage

ELISA

1:8000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 31750298). IHC: NBP1-05170 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP1-05170 is more sensitive than NBP2-23522 or NBP2-23523 in ELISA. (2) IP: NBP1-05170 can be used in immunoprecipitation.

Reviewed Applications

Read 1 review rated 5 using NBP1-05170 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Endorepellin/Perlecan

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodeling (2).

Long Name

Heparan Sulfate Proteoglycan

Alternate Names

HSPG2, Perlecan

Gene Symbol

HSPG2

Additional Endorepellin/Perlecan Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free

Customer Reviews (1)

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  • Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free
    Name: KARTHIKEYAN THIRUGNANAM
    Application: Immunocytochemistry
    Sample Tested: Brain microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 01/23/2024
    Human brain microvascular endothelial cells stained for PDGF-BB (Red) and HSPG (Green) and nuclear DAPI (Blue) show the endothelial deposition of basement membrane molecule, HSPG.
    Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free NBP1-05170

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