Endosialin/CD248/TEM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77310

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Endosialin/CD248 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The immunogen is located within the last 50 amino acids of TEM-1.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Specificity

Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77310 can detect all isoforms of human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46, 81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endosialin/CD248/TEM1 Antibody - BSA Free

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]

Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 4357, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310]

Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310]

Immunohistochemistry: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human Colon tissue with TEM1 antibody at 20 ug/mL.
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]

Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/mL.
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]

Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77310]

Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 (NBP1-77310), (2 ug/mL), TEM-1 (NBP1-77311), (1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Endosialin/CD248/TEM1 Antibody - BSA Free

Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310] -

Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310] - Validation of TEM-1 in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Endosialin/CD248/TEM1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310] -

Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310] - Figure 5 Immunofluorescence Validation of TEM-1 in Human Colon Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for Endosialin/CD248/TEM1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30285896).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Endosialin/CD248

Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.

Alternate Names

CD164L1, CD248, TEM1

Entrez Gene IDs

57124 (Human); 70445 (Mouse); 293669 (Rat)

Gene Symbol

CD248

UniProt

Q9HCU0 (Human)

Additional Endosialin/CD248 Products

Product Documents for Endosialin/CD248/TEM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Endosialin/CD248/TEM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endosialin/CD248/TEM1 Antibody - BSA Free

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Protocols

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