Endosialin/CD248/TEM1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77311
![Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]](https://resources.rndsystems.com/images/products/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0005.jpg "Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TEM-1. The immunogen is located within amino acids 40-90 of TEM-1
Specificity
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD), and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and Rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77311 can detect human isoform 1, mouse and rat TEM-1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Endosialin/CD248/TEM1 Antibody - BSA Free
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.![Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]](https://resources.rndsystems.com/images/products/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0002.jpg "Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]")
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml.![Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]](https://resources.rndsystems.com/images/products/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0004.jpg "Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]")
Western Blot: Endosialin/CD248/TEM1 AntibodyBSA Free [NBP1-77311]
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77310, (2 ug/mL), TEM-1 NBP1-77311, ( 1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] -
Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] - Figure 4 Immunohistochemistry Validation of TEM-1 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] -
Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] - Validation of TEM-1 in Human Colon Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).Applications for Endosialin/CD248/TEM1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Endosialin/CD248
Additional Endosialin/CD248 Products
Product Documents for Endosialin/CD248/TEM1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Endosialin/CD248/TEM1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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