Enolase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03484

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Enolase 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human Enolase 1 (NP_001419.1). MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Enolase 1 Antibody - BSA Free

Western Blot: Enolase 1 AntibodyAzide and BSA Free [NBP3-03484]

Western Blot: Enolase 1 AntibodyAzide and BSA Free [NBP3-03484]

Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of U-2 OS cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunohistochemistry-Paraffin: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Analysis of 200ug extracts of HeLa cells using Enolase 1 antibody. Western blot was performed from the immunoprecipitate using this antibody.
Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of NIH-3T3 cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunohistochemistry-Paraffin: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Paraffin-embedded human colon carcinoma using Enolase 1 antibody at dilution of 1:100 (40x lens).
Knockout Validated: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Western Blot: Enolase 1 Antibody - Azide and BSA Free [NBP3-03484]

Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using Enolase 1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for Enolase 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50-1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Enolase 1

Enolase 1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

Additional Enolase 1 Products

Product Documents for Enolase 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Enolase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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