Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Amphibian, Bovine, Canine, Drosophila, Sheep

Cited:

Human, Mouse, Rat, Porcine, Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all eNOS Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues S(1179) LQERQLRGAVPWAFD(1194) of human eNOS.

Epitope

SLQERQLRGAVPWAFD

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 31352175).

Specificity

This does not detect human inducible NOS or rat brain NOS.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit eNOS Antibody - BSA Free (NB300-500) is a polyclonal antibody validated for use in IHC, WB and Flow. Anti-eNOS Antibody: Cited in 28 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for eNOS Antibody - BSA Free

eNOS Antibody - BSA Free-Western Blot-NB300-500-img0007.jpg
Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500]

eNOS-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB300-500-img0006.jpg
Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry-Paraffin: eNOS Antibody - BSA Free [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
Immunohistochemistry: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry: eNOS Antibody - BSA Free [NB300-500]

Immunohistochemistry: eNOS Antibody - BSA Free [NB300-500] - Staining of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group. Dark brown staining of indicates eNOS-positive cells. Magnification x400.
eNOS Antibody - BSA Free

Immunohistochemistry: eNOS Antibody - BSA Free [NB300-500] -

Expression of endothelial markers indicate functional endothelium in TEVG.A. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). B. Real-time PCR analysis of ephrinB2 and eNOS. Ephrin-B2, a marker of arterial vessels. (n = 5–10 in each group)
eNOS Antibody - BSA Free

Western Blot: eNOS Antibody - BSA Free [NB300-500] -

Western Blot: eNOS Antibody - BSA Free [NB300-500] - Mevalonate pathway inhibitors reversed fractionated-radiation–induced suppression of KLF2 & its downstream target molecules. Representative Western blot analysis & quantification of KLF2, TM, & eNOS 4 h after exposure to five fractions of 2 Gy (a) in presence or absence of atorvastatin (1 μM) or GT3 (5 μM) & (b) in presence or absence of GGTi (10 μM) (n = 3). beta -actin served as a loading control. (n, number of independent experiments performed; a, significant statistical difference between nonirradiated & irradiated groups; b, significant statistical difference between fractionated irradiation & single exposure; *, p < 0.05; **, p < 0.01; ***, p < 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
eNOS Antibody - BSA Free

Western Blot: eNOS Antibody - BSA Free [NB300-500] -

Western Blot: eNOS Antibody - BSA Free [NB300-500] - Upregulation of HMG-CoA reductase in shPLD2 cells decreases eNOS expression. (A) Representative western blot of HMG-CoA reductase in Scramble & shPLD2 cells. The regions of the Western blots containing the HMG-CoA reductase & actin immunoreactive bands were scanned & the relative abundance of the individual samples quantified using an Odyssey CLx imaging system (B) Quantification of western blots with HMG-CoA reductase normalized to actin (n = 4 experiments). (C) N-SIM microscopy of HMG-CoA reductase; Bar, 7.5 μM. (D) RT-PCR of HMG-CoA reductase in Scramble & shPLD2 cells (n = 3). (E) Western blot of eNOS after treatment with NFOT & Simvastatin. Representative image of 3 experiments. The regions of the Western blots (Suppl. Figure 2) containing the eNOS & actin immunoreactive bands were scanned & the relative abundance of the individual samples quantified using an Odyssey CLx imaging system. (F) Quantification of western blots with eNOS levels normalized to actin (n = 3). Comparisons without bars made to untreated Scramble values. (G) eNOS activity after treatment with Simvastatin (n = 7). Mean ± SEM; *p < 0.05; **p < 0.01; ***p ≤ 0.001; One-way ANOVA with Bonferroni’s Multiple Comparison Test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28831159), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
eNOS Antibody - BSA Free

Western Blot: eNOS Antibody - BSA Free [NB300-500] -

Western Blot: eNOS Antibody - BSA Free [NB300-500] - The human PLD2 polymorphism R172C does not alter eNOS signaling but does decrease caveolin-1 protein levels. Overexpression of HA-tagged hPLD2 (A) & HA-R172C-PLD2 (B) in HeLa cells, visualized using anti-HA immunofluorescent staining (green). Arrowhead, PLD2 localization in filopodia; chevron, in peripheral actin ruffles; *, in dorsal actin ruffles; arrow, in subcortical actin network. Bar, 7.5 μM. Representative image of multiple experiments. (C) Representative western blot of eNOS after transfection of HA-hPLD2 or HA-R172C-PLD2 into shPLD2 cells. The regions of the Western blots containing the eNOS & actin immunoreactive bands were scanned & the relative abundance of the individual samples quantified using an Odyssey CLx imaging system. (D) Quantification of western blotting with eNOS levels normalized to actin (n = 3). (E) eNOS activity as measured by nitrate formation (n = 7). Lane 1 vs 2, P = 0.0021; lane 2 vs 3, P = 0.00035; lane 2 vs 4, P = 0.00012. (F–I) N-SIM microscopy of plasma membrane eNOS & caveolin-1. Bar, 7.5 μM. Representative image of multiple Scramble or shPLD2 cells imaged. Cells in H & I were selected for imaging based on expression of HA-hPLD2 or HA-R172C-PLD2 as visualized by anti-HA immunofluorescence in a separate channel (not shown). Mean ± SEM; *p < 0.05; **p < 0.01; ***p ≤ 0.001; One-way ANOVA with Bonferroni’s Multiple Comparison Test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28831159), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for eNOS Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:50-1:1000
Application Notes
In IHC, antigen retrieval is not essential but may optimize staining. By Western blot, a 140kDa band is seen in human endothelial cells. Optimal dilutions should be determined by the end user.

Reviewed Applications

Read 3 reviews rated 4.7 using NB300-500 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eNOS

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Long Name

Endothelial Nitric Oxide Synthase

Alternate Names

NOS3

Entrez Gene IDs

4846 (Human); 18127 (Mouse); 24600 (Rat)

Gene Symbol

NOS3

UniProt

P29474

Additional eNOS Products

Product Documents for eNOS Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for eNOS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

Citations for eNOS Antibody - BSA Free

Customer Reviews for eNOS Antibody - BSA Free (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • eNOS Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 05/23/2017
  • eNOS Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 05/23/2017
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse Carotid Artery
    Species: Mouse
    Verified Customer | Posted 02/22/2016
    eNOS antibody tested on mouse carotid artery
    eNOS Antibody - BSA Free NB300-500

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Protocols

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